Modified Herpes Virus Keeps Arteries ‘Free-flowing’ Following Procedures

July 11, 2007

Christopher Skelly, MD, assistant professor of vascular surgery at the University of Chicago Medical Center, and the study’s lead author says, “One of the drawbacks of balloon angioplasty to open blocked arteries and the use of stents to keep them open is that arteries sometimes experience aggravation from the procedure. The balloon angioplasty, in addition to opening the artery can lead to smooth muscle cell proliferation, similar to formation of scar tissue, known as neointimal hyperplasia. This scar tissue can restrict blood flow not long after the procedures, leading to a recurrence of symptoms. A significant number of these cases end up requiring further intervention to address this complication.”

Researchers at the University of Chicago noted that in recent years, genetically engineered herpes simplex virus studied for its efficacy against malignant tumors of the central nervous system and the liver was blocking certain types of cell death and proliferation of surviving cells. They wanted to test this effect in arteries following angioplasty therapy.

The researchers studied a rabbit model that replicates the restenosis or renarrowing after angioplasty. Rabbits that underwent angioplasty alone experienced significant narrowing of the artery. Rabbits exposed to the herpes simplex virus during angioplasty had minimal changes in the arteries. The smooth muscle cell proliferation which causes the restenosis was very low in the group treated with herpes and remained high in the untreated group.

One undesirable, yet expected outcome of angioplasty and stent placement is the disruption of the artery’s endothelial layer, which forms the inner lining of the artery. Loss of this inner layer predisposes the artery to blood clot formation which has been a recent concern with drug eluting stents. The researchers found that the endothelial layer was partially restored at 14 days and completely restored at 28 days post-balloon angioplasty in the group treated with the herpes virus.

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“The ability to target the smooth muscle cells that cause the narrowing, and regenerate the endothelial cell lining is an important finding,” noted Skelly.

“This study is an important step in the application of genetically engineered herpes simplex viruses for treatment of vascular disease,” Skelly added. “It suggests that genetically engineered viruses may have a significant impact on the outcomes of angioplasty performed in humans. Human trials would be the next step to test this theory.”

The study was supported by the American Heart Association and the American College of Surgeons. Additional authors include Amito Chandiwal and James E. Vosicky from vascular surgery; Ralph R. Weichselbaum, from radiation and cellular oncology; and Barnard Roizman, from Marjorie B. Kovler Viral Oncology Laboratories, all at the University of Chicago

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Bringing together opposites: how dialogue groups are helping to reduce HIV risk behaviour in Africa and Miami

July 9, 2007

Studies of HIV prevention interventions from remote villages in South Africa and from the ‘gay ghetto’ of Miami, presented at the Eighth AIDS Impact Conference in Marseilles last week, show that facilitating dialogue between potential transmitters and acquirers of HIV who normally don’t talk together – in one case young men and women, in the other HIV-positive and negative gay men – can produce significant reductions in risk behaviour and in HIV/STI incidence.

South Africa: The Stepping Stones Study

Stepping Stones is not a new programme. Devised in 1995 by Alice Welbourn, Chair of the International Community of Women Living with HIV (ICW), it is described as a “gender transformative programme for HIV intervention,” in other words it uses participatory learning groups to bring young women and men together to talk about issues such as sexual violence, women’s inequality, negotiating condom use and the way gossip is used to destroy reputation.

Introduced in Uganda, it was brought to South Africa in 1998, but this trial was the first proper evaluation of its biological and behavioural outcomes. Direct measurements of HIV and STI incidence are quite rare in studies of behavioural interventions, as they require large study groups in high-incidence areas. Most studies measure behavioural outcomes.

Rachel Jewkes of the South African Medical Research Council told the conference that Stepping Stones was probably the most widely-used HIV behavioural intervention in the world. Other countries that have used it include Tanzania and Mozambique, where 500,000 young people have been through the programme.

The intervention consists of 14 three-hour single-sex groups for young women and men, who are then brought together for three mixed-sex dialogue groups. This controlled trial consisted of comparing the full Stepping Stones programme with one called “Stepping Stones Short” which included the modules on HIV information, safer sex and condom use but omitted the broader discussions of gender relations.

The trial recruited 1423 women and 1371 men aged 15-26 years from 70 villages in a remote rural region of Eastern Cape province around the town of Umtata. Villages, rather than individual participants, were randomised to receive either the full or the short programme.

Most of the young people were recruited through schools. HIV and herpes (HSV-2) prevalence and sexual behaviour were assessed at baseline, 12 months and 24 months through blood tests and in a face to face interview. Follow-up rates at 24 months were 87% (for blood tests) and 75% (for the behavioural re-interview). All 70 villages stayed in the programme.

A small qualitative study interviewing 11 women and 10 men before and after the study elicited more in-depth reactions to the programme.

At baseline 11% of the women and 2% of the men had HIV, and 27% and 10% respectively had HSV-2. Incidence of HSV-2 was measured because HSV-2 infection increases vulenrability to HIV infection due to genital herpes ulcers, and because HSV-2 infection, even without symptomatic genital herpes, may increase HIV shedding in genital fluids, particularly in women.

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At follow-up there was no difference in HIV incidence amongst the men (1.38% a year in both arms) and only a non-significant difference in herpes incidence (1.42% versus 2.02%, p = 0.36), though Jewkes commented that HIV incidence was generally lower than expected. In women there was a non-significant difference in HIV incidence (5.69% versus 6.74%, p = 0.35), but a significant difference in herpes acquisition (5.1% versus 7.4%). Adding together HIV and HSV-2 incidence in both women and men yielded a ‘composite efficacy’ of 31% for the programme.

There were significant behavioural changes amongst the men. Men in the full programme reported 9% fewer sexual partners at 12 months and 14% fewer at 24 months and also reported more condom use, less transactional sex, less severe inter-partner violence, and less substance use. Forty-four per cent of men in the intervention arm decided to get an HIV test versus 34% in the control arm.

There were fewer differences in behavioural outcomes for women, which may reflect less ability to take control over behaviour. Jewkes told the conference that young men showed increased rates of carrying condoms (even though one man described doing this as “hunting with salt”, i.e. appearing over-ready for sex) and using them.

Women however showed less sense of agency in their dealings with men; one young woman described her experience of unsafe sex with an older boyfriend (a teacher) as “these things happen on their own,” and although some women were able to insist on condom use, others described not daring to for fear of jeopardising their relationship.

Miami: the MensROOM Study

The intervention in Miami, in contrast to Stepping Stones, was a pilot intervention of a new concept in HIV prevention for gay men in which ‘high-risk’ HIV positive and HIV negative gay men were brought together to discuss HIV risk and risk behaviour.

Researcher Steven Kurtz of the University of Delaware first conducted extensive focus groups among gay men described as heavy substance users (meaning more than three instances of recreational drug use in the last 90 days, excluding alcohol) and having significant amounts of unprotected sex (meaning more than two incident in the last 90 days).

This fieldwork indicated that HIV risk was exacerbated, said Kurtz, “because of striking differences in HIV-positive and HIV-negative men’s attitudes toward the severity and meaning of HIV infection, their perceptions of responsibility for self protection and disclosure, and the lack of shared meanings of non-verbal serostatus disclosure techniques.”

He told the conference: “Safer sex behaviour and serostatus disclosure norms are different for HIV positive and negative men.”

Because of this, he came to the conclusion that conducting discussion or support groups restricted to men of one HIV status may do more harm than good.

“Serostatus-segregated risk reduction interventions maintain social segregation,” he told the conference, “and may sustain bounded disclosure norms.”

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The Bachelor Producers Hit With Herpes Dilemma.

July 2, 2007

The Bachelor Producers Hit With Herpes Dilemma…. The producers of U.S. reality TV show The Bachelor have been floored by the amount of pretty women who don’t get to woo series hunks - because they have herpes.

A new report reveals a substantial number of single stunners were turned away from the most recent show’s auditions after testing positive for herpes and other communicable diseases.

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A show insider tells the National Enquirer, “Some of the best looking women have been told recently that they didn’t pass the medical portion of the test due to herpes.”

The most recent Bachelor was U.S. Navy Lieutenant Andy Baldwin.

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Hello stranger, are you sick enough for me?

June 25, 2007

AUSTRALIAN lonely hearts with health conditions such as cancer, herpes, irritable bowel syndrome and allergies are turning to a specialised online dating service in their quest to find love.

The website Prescription4Love is the brainchild of American Ricky Durham, 46, who was inspired by his late brother Keith’s struggle to meet new people while he suffered from Crohn’s disease. Keith died in 2004.

“It was hard for him to disclose his disease to anyone, but it was really hard for him to tell someone he had a colostomy bag,” Mr Durham said.

“When do you tell someone that you have a colostomy bag? The first time you meet? The first date? The second? So I thought if he met someone at a website where everyone had the same condition, there would be nothing to disclose.”

The free site is one of a growing number of dating and friendship services specialising in health conditions, with sexually transmitted infections now leading the way.

US-based websites such as Positive Singles and Antopia, a herpes group claim to have tens of thousands of infected members and boast hundreds of “success stories”.

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Mr Durham, from Atlanta, Georgia, said he began Prescription4Love for sufferers of 11 conditions, including cancer, obesity, deafness, herpes, HIV, diabetes, Crohn’s disease and allergies. At the request of clients, he has recently expanded the site to include those with less common conditions such as epilepsy, paraplegia and human papillomavirus, which causes genital warts and cervical cancer, as well as transplant patients and burns victims.

The site has about 1000 members mostly in the US, but Mr Durham said people from Australia, Canada, Britain and Israel had also joined up.

Clients include “Sweet Lady”, 24, from Oklahoma, who’s deaf and “looking for a date or making friends” and Kelvin, 45, from Pennsylvania, a recovering alcoholic with diabetes “looking for love”.

Mr Durham, who had no previous experience in IT said honesty was important in a relationship, but finding an opportune time and situation to raise such subjects as medical conditions, particularly embarrassing ones, could be difficult.

“By using Prescription4Love.com, you can be honest in advance,” he said.

Lija Jarvis from mainstream Australian online dating website RSVP said that, while it doesn’t have any services catering for disabled people, this has been identified as a potential need.

She said the feature might be built into sub sites in the future.

RSVP is owned by Fairfax Media, publisher of smh.com.au and theage.com.au.

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Herpes No. 1 Virus Impacting, Killing Corals

June 21, 2007

Only, for corals, a herpes virus infection isn’t just annoying.

It can be lethal, and it and other diseases are possibly a big factor in the deaths of coral reefs that humans are causing throughout the world’s oceans, new research shows.

Scientists have known for years that humans are killing corals indirectly and directly through global warming, overfishing and pollution.

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Many reefs off populous coasts have been decimated, while those near uninhabited areas are often thriving.

“For some reason, when you put people next to reefs, they die,” said microbiologist Forest Rohwer of San Diego State University at a recent symposium at the American Museum of Natural History here.

A 2004 study found that 70 percent of the world’s reefs had been destroyed or were threatened by global warming and other human activities.

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Herpes Virus Hijacks DNA Repair Process

June 14, 2007

The DNA damage response normally fixes DNA errors caused by radiation or other environmental factors, or mistakes accidentally introduced when cells copy their genetic material prior to dividing.

In the murine (or mouse-infecting) herpes virus they studied, researchers at Washington University School of Medicine in St. Louis identified a protein that can trick the mouse cell into turning on the DNA damage response. They also showed that Epstein-Barr virus, a human herpes virus, has a similar protein. Scientists found that blocking murine herpes virus from activating the DNA damage response caused viral replication rates to plummet.

“We don’t want to treat viral infection by blocking DNA damage response systemically because this process is used constantly throughout the body and is very important to preventing cancer,” says lead author Vera Tarakanova, Ph.D., a postdoctoral fellow. “However, by targeting the viral protein responsible for activating the DNA damage response, we may be able to block viral replication. In addition, determining how the DNA repair response helps viral replication may enable us to develop novel strategies to treat infection.”

The paper appears online this week in Cell Host & Microbe. Scientists have known for some time that viral infection of cells activates the DNA damage response. But researchers had assumed that this activation occurred because repair mechanisms were mistaking replicating viral DNA for damaged or dysfunctional cellular DNA.

“Viruses sometimes structure their own DNA differently than cellular DNA,” Tarakanova notes. “Many of us thought that such differences might be triggering the DNA damage response.” Working in the laboratory of Herbert W. “Skip” Virgin, M.D., Ph.D., Edward Mallinckrodt Professor and head of the Department of Pathology and Immunology, Tarakanova found that murine herpes virus, rather than the host cell, was triggering the DNA damage response. She showed that introducing just one viral protein into cells led to activation of two cellular proteins involved in the damage response, ATM and H2AX.

The viral protein that triggered this inappropriate activation, orf36, is a kinase, a type of protein that chemically modifies other molecules to activate different processes or transmit signals. Genetic comparisons with several human herpes viruses revealed kinases similar to orf36 in the human viruses. Scientists then took a similar kinase from human Epstein-Barr virus and showed that introducing it into cells also activated the DNA damage response.

When the research team genetically disabled orf36 in the murine herpes virus and infected mouse cells with it, the virus no longer activated the DNA damage response. The virus’s ability to replicate also dramatically decreased.

Kinases are versatile proteins that sometimes play multiple roles. To ensure that enhanced viral replication wasn’t linked to orf36’s interactions with other molecules, researchers turned to mice lacking the genes for ATM and H2AX, the damage response proteins activated by infection. When they infected cells from these mice with murine herpes virus, its ability to reproduce was again curtailed. How DNA damage response benefits viral replication is still a mystery and a topic of continuing investigation in the Virgin lab.

“The discovery that induction of the cells’ DNA damage response is an intentional viral strategy, rather than a passive cellular response to viral invasion, means that we should look into whether other DNA viruses use a similar approach to enhance their growth,” says Virgin.

Reference: Tarakanova VL, Leung-Pineda V, Hwang S, Yang C-W, Matatall K, Basson M, Sun R, Piwnica-Worms H, Sleckman BP, Virgin HW IV. Gamma-herpesvirus kinase actively initiates a DNA damage response by inducing phosphorylation of H2AX to foster viral replication. Cell Host and Microbe, online edition.

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Manson Amongst Prisoners Released With Herpes

June 13, 2007

Corcoran State Prison, CA –Convicted mass-murderer Charles Manson has today been released from Corcoran State Prison for medical reasons, but will be confined to his home with an electronic ankle bracelet for 48 years.

Reggie Dunbar, spokesperson from the prison, said that the California District Attorney’s office granted Manson’s release shortly after it was informed he had had a herpes rash that looked like a “swastika” on his forehead.

“Poor Charlie, he was really embarrassed, I can’t imagine the emotional scars this has left him with,” Dunbar added.

The release of Manson has followed a mass exodus of prisoners from California prisons in what has been called, “The Herpes Defense.”

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Dunbar stated, “Once prisoners found out that a case of Herpes could get them them transferred to a home detention, the California prison population had a herpes outbreak that would rival USC’s Fraternity Row, both of which came from the same source.”

According to Dr. Reggie Athansoulis for the Center of Disease Control, herpes traditionally effects one in eight Americans, or basically everyone in California.

“It’s not life threatening, “Athansoulis said, “but it certainly threatened the show, ‘The Simple Life,’ where it originated.”

The Herpes Defense has now become a part popular culture, with reports of it being used in every day life. Reggie Stanthorpe, from Media watchdog group, Media Connection UK recently said, “Herpes is like a modern day Moses, leading prisoners out of prison, getting people out of traffic fines, excusing kids for late assignments. It’s no longer acceptable to blame traffic or ‘the baby’ for running late for social functions, but it’s perfectly acceptable now to blame herpes.”

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Herpes awareness campaign kicks off

June 6, 2007

Saying African-Americans face a disproportionally high incidence of genital herpes, a major drug maker and several health groups will launch a public awareness campaign today in Detroit.

Local radio and print ads are designed to educate people about the sexually transmitted disease, which can cause painful blisters and is not curable. The sponsors, led by Glaxo-SmithKline, will roll out future campaigns in Atlanta and Baltimore.

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“Herpes has always been one of those STDs that can be treated but never goes away,” said Dr. Peter Gulick, an East Lansing doctor and associate professor at Michigan State University. “You can get a reactivation years later, even if you don’t have sexual activity.”

About 40 percent of black adults in the United States have genital herpes — compared with 14 percent of whites and 12 percent of Mexican-Americans, according to an August 2006 study by the Centers for Disease Control and Prevention.

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All you need to know to give your smile the white stuff

June 5, 2007

A toothache can lead to headaches, loss of appetite and a bad attitude.

Chapped lips aren’t only uncomfortable, but unattractive.

And a canker sore is about as pleasant as an enema.

If our mouths aren’t feeling well, we’re not feeling well.

“The mouth, teeth, lip and gums are important for maintaining health, and they are also markers of overall health,” said University of California, Irvine Medical Center’s Dr. William B. Armstrong, who specializes in head and neck cancer surgery. “If someone is malnourished, the gums and teeth will show it. Poor oral care goes along with chronic disease.”

The mouth and lips are also key to an attractive appearance.

“Everyone always remembers someone’s smile,” said Anastazia Grey Woodin, a makeup artist and esthetician at Baker Street West Salon in Costa Mesa, Calif.

Filling-free and healthy

Eat a balanced diet, following the five major food groups (breads/grains; fruit; vegetables; dairy; meats/fish) to ensure you are getting all the nutrients your body needs to build and maintain strong bones, decay-resistant teeth and healthy gums, Dr. Nick Salvati, who owns a private dental practice in San Clemente, Calif., said.

Visit your dentist at least twice a year, said Salvati. And don’t forget to brush your teeth two to three times a day and floss.

Inspect your teeth and mouth for changes in gum color, bleeding areas, sores, or cuts on the gums or tongue whenever you brush, said Dr. Maged Zakhary, president of TruDental in Newport Beach, Calif.

Use an American Dental Association-approved fluoride toothpaste like Colgate Total, Biotene (for dry mouth) or Sensodyne (for sensitive teeth), Salvati said. Be careful about using whitening and tartar-control toothpastes since their additives can make teeth very sensitive. Though both types of toothpaste are safe for the most part, peroxide in whitening products and phosphate in tartar-control toothpastes can lead to sensitivity to temperature and peeling of mouth tissues.

Drink more water and cut down on soda, said dentist Dr. Pooneh Sabo, owner of OC Dental Center in Santa Ana, Calif. When you do drink soda, use a straw so the liquid stays in your mouth less. Dilute juices with water.

Watch your milk intake, Sabo said. Milk has a lot of sugar in it, so brush or rinse after drinking.

Gorgeous lips, nice smile

Avoid drawing too much past your lip line when applying color; overdraw only to correct the shape of your lips, Woodin said. When applying color, start your liner or lip brush at the side of your top lip and move upward.

Apply a few drops of peppermint or tea tree oil to your tongue or toothbrush to help freshen your breath and kill bacteria, UC Irvine dermatologist Dr. Christopher B. Zakhary said.

To keep your lips soft and supple, use lip balms that contain soothing, moisturizing ingredients like cocoa or shea butter, aloe vera, maple (an antioxidant), tea tree and/or vitamin E, said Tamara Freedman, esthetician and director of education at Spa Gregorie’s in Newport Beach, Calif.

Avoid ingredients like camphor and menthols because they will dry out your lips, causing you to use more and more product. Don’t forget to get a lip balm with sun protection, said Zachary.

For fuller lips, consider hyaluronic acid fillers like Restylane and Juvederm, Zachary said. Fillers generally last three to nine months, though some companies are making ones that last a few years.

Use glycolic acid products around the lips twice a day to exfoliate the area and make the skin look smoother, said Dr. Valdemar Ascencio, a plastic surgeon at Saddleback Memorial Medical Center in Laguna Hills, Calif.

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ORAL FRUSTRATIONS

Orange County, Calif.-area dentists Dr. Nick Salvati and Dr. Pooneh Sabo, and head/neck cancer surgery specialist Dr. William B. Armstrong offer their insight on a few common mouth conditions.

Canker sores: A recurring problem for 20 to 50 percent of the population, canker sores may be caused by a bacterium that lays dormant in the salivary glands, but releases toxins during times of mental and physical stress, trauma to the mouth, menstruation, etc., Salvati said. The result is a painful ulcer often the size of a dime on the soft mucosa (cheeks, inner lip, floor of the mouth, side of the tongue) inside the mouth.

Certain spicy foods can irritate an existing ulcer as well as cause a new one to form. While canker sores typically heal on their own, you can try using over-the-counter numbing treatments like Anbesol or prescription medications that can reduce pain and healing time.

Prevention tips include reducing stress and chances for oral trauma (for instance, limit gum chewing to prevent accidental cheek biting). Vitamin supplements like lysine may also help.

Cold sores: Cold sores, or fever blisters, are a recurring problem for about 40 percent of the population, Salvati said. They’re caused by the Herpes Simplex Virus (HSV), which is believed to be transmitted through respiratory secretions like coughing and sneezing, sexual or physical contact, and through the placenta at birth.

Cold sores typically appear as single or small groups of painful blisters on the roof of the mouth, gums, lips and nose. They can recur on a regular basis or randomly.

Treatment options include prescription antiviral medications and laser therapies. To prevent them, avoid eating or drinking from other people’s utensils or cups. Also, avoid touching the sore so you don’t spread it to other people or to other areas of your body, like your eyes.

Oral cancer: Cancer in the oral cavity can look like a white or red lesion, Armstrong said. It is often painless, but can also be painful and may bleed. You may think you bit your lip or tongue, but it doesn’t heal within a week or two.

Risk factors for developing oral cancer include cigarettes, chewing tobacco and alcohol. Treatment options depend on the extent of the tumor, and include surgery or radiation therapy.

Gum disease: Occurring in about 80 percent of Americans, gum disease is an inflammation of the gums, Sabo said. Since there’s often no pain associated with gum disease, many people have it without knowing it.

Symptoms can include red and inflamed bleeding gums, and foul odor in the mouth. In more advanced cases, teeth can become loose. Gum disease is caused by plaque and bacteria building up under the gums.

Treatment options include basic dental cleaning, deep cleaning, bone grafts to stabilize loose teeth and/or gum grafts.

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Human Herpes Virus 6B Is Associated With Mesial Temporal Lobe Epilepsy

May 31, 2007

There is strong evidence that one particular type of epilepsy is associated with a viral infection, according to new research. The international group of researchers, led by Steve Jacobson from National Institute of Neurological Disorders and Stroke, USA, found DNA from the virus, Human Herpes Virus 6B (HHV-6B) in specific regions of the brains in 11 of 16 patients with mesial temporal lobe epilepsy (MTLE) referred for investigation compared with zero of seven (0%) patients without MTLE.

MTLE is a common, severe, type of epilepsy that usually begins in childhood. Mesial temporal sclerosis is a change often seen in the brains of patients with this form of epilepsy. Temporal lobectomy is often used to control MTLE. HHV6 is usually acquired in early childhood - more than 90% of the general population can be shown to have been infected with the virus. After primary infection, HHV-6 can persist lifelong in some white blood cells, salivary glands, and in the central nervous system. Two HHV-6 variants are known, HHV-6A and HHV-6B. Active infection or reactivation of HHV-6 in the brain has been previously shown to be associated with neurological disorders, including epilepsy and encephalitis.

As well as showing the presence of viral DNA in patients with MTLE, in additional studies in one patient who had surgery over many months because of recurrent epilepsy, the researchers were able to detect an antigen (HHV-6 gp116/54/64), which is specific for HHV-6B, in astrocytes (primary brain cells). Finally, the authors showed that infection of cell cultures of astrocyes infected with HHV-6 showed a marked decrease in the expression of one protein, EAAT, which is known to be involved in transmitting glutamate across cell membranes; glutamate is a substance that acts to carry signals within the brain.

Overall, the researchers have now have detected HHV-6B in 15 of 24 patients with mesial temporal sclerosis or MTLE, in contrast to zero of 14 with other syndromes. If these findings are confirmed in other groups of patients with this form of epilepsy, it may open up new avenues of therapy.

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